Chemical and Thermal Characterization of an exopolysaccharide from Lactiplantibacillus plantarum BAL-29-ITTG

Rony Obed Suchiapa Diaz; Lucia Maria Cristina Ventura Canseco; Alejandro Ramírez Jiménez

doi:10.37636/recit.v8n4e405

Autores/as

Rony Obed Suchiapa Diaz Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana, km. 1080. C.P. 29050, Tuxtla Gutiérrez, Chiapas, México.
Lucia Maria Cristina Ventura Canseco Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana, km. 1080. C.P. 29050, Tuxtla Gutiérrez, Chiapas, México. https://orcid.org/0000-0003-3700-9749
Alejandro Ramírez Jiménez IIXM SECIHTI-Tecnológico Nacional de México/Instituto Tecnológico de Tijuana, Blvd. Industrial s/n, Cd Industrial, 22430 Tijuana, Baja California, México. https://orcid.org/0000-0002-3011-1612

DOI:

https://doi.org/10.37636/recit.v8n4e405

Palabras clave:

Exopolisacáridos, Determinación de monosacáridos, resonancia magnética nuclear, Composición de polisacáridos, Caracterización de polisacáridos

Resumen

– Los exopolisacáridos (EPS) son biopolímeros que pueden ser producidos por bacterias ácido lácticas, En este trabajo, un EPS proveniente de Lactiplantibacillus plantarum BAL-29-ITTG fue caracterizado mediante resonancia magnética nuclear (RMN) de ¹H, ¹³C, COSY, TOCSY y HSQC, espectroscopía de infrarrojos (FTIR) calorimetría diferencial de barrido (DSC), análisis termogravimétrico (TGA) y viscosimetría. Los resultados de análisis térmicos y viscosimetría indican que este EPS tiene una estructura ramificada y una masa molar alta; para determinar los monosacáridos principales, los desplazamientos químicos de carbono e hidrógeno obtenidos mediante RMN fueron cargados y comparados con la base de datos del software en línea CASPER: http://www.casper.organ.su.se/casper/ Los resultados mostraron que, al menos, ocho monosacáridos diferentes componen este EPS, los más probables identificados fueron: b-D-glucosa con uniones 1-4 y 1-6: →4)-b-D-Glc-(1→; →6)-b-D-Glc-(1→ y a-D-manosa con uniones 1-3, 1-4 y 1-6: →3)-a-D-Man-(1→; →4)-a-D-Man-(1→ y →6)-a-D-Man-(1→, aunque los datos de FTIR y RMN sugieren también la presencia de residuos N-acetilados.

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