Automation of inoculation in culture media for the microbiology laboratory
DOI:
https://doi.org/10.37636/recit.v6n4e285Keywords:
Clinical laboratory, Streak, Culture sowing, Microorganisms, Cross contaminationAbstract
The clinical laboratories belonging to the health system in Mexico are among the most discriminated departments due to factors such as sources of financing, as it requires strong administrative management, as well as the dependence on vast infrastructure, material, and human resources. Pneumonia, a disease that can be detected in a subclinical stage, is the 2nd cause of death from respiratory infections in Mexico and is, in turn, the 7th cause of death in the country. For the early diagnosis of diseases caused by microorganisms such as the one just mentioned, laboratory tests are performed, including inoculation in culture media for the proliferation of said microorganisms, however, the techniques used are manual, delaying technological progress. applied in terms of automation, which would allow better workflows with fewer resources. A great challenge in automating the process is cross-contamination that occurs at the time of inoculation, as well as decontamination and sterilization after sowing. Considering this, the model of a device that performs the microbiological culture process automatically was developed, emphasizing the variables involved to avoid cross-contamination and sterilization of the areas, comparing various decontamination techniques, the ones that best fit us are through exposure to UV light, since we avoid the malfunction of electronic components due to temperature.
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J. R. Pérez-Padilla. “Muertes respiratorias en México, 2015”, NCT Neumología y Cirugía de Tórax, vol. 77, no 3, pp. 198-202, 2018. ISSN 2594-1526. Disponible en: https://www.medigraphic.com/pdfs/neumo/nt-2018/nt183e.pdf
Instituto Nacional de Estadística y Geografía. “Principales causas de muerte desglosadas por sexo Enero – junio 2021”, Comunicado de prensa num 24/22. INEGI 2021. Disponible en: https://www.inegi.org.mx/contenidos/saladeprensa/boletines/2022/dr/dr2021.pdf
P. Jiménez y A. Calvo. “Diagnóstico microbiológico de la neumonía del adulto adquirida en la comunidad”. Revista Chilena De Enfermedades Respiratorias, vol. 21, no. 2, pp. 95-101, Abril 2005. http://dx.doi.org/10.4067/S0717-73482005000200005 DOI: https://doi.org/10.4067/S0717-73482005000200005
C. M. C. Rodríguez and H. Groves. “Community-acquired pneumonia in children: the challenges of microbiological diagnosis”. Journal of Clinical Microbiology, vol. 56, no. 3, pp. 10-1128, Febrero 2018. https://doi.org/10.1128/jcm.01318-17 DOI: https://doi.org/10.1128/JCM.01318-17
E. Hervé. “Nuevas tecnologías en diagnóstico microbiológico: automatización y algunas aplicaciones en identificación microbiana y estudio de susceptibilidad”. Revista Médica Clínica Las Condes, vol. 26, no. 6, pp. 753-763, Noviembre 2015. https://doi.org/10.1016/j.rmclc.2015.11.004 DOI: https://doi.org/10.1016/j.rmclc.2015.11.004
J. P. Hays and W. B. Van Leeuwen (eds.). “The role of new technologies in medical microbiological research and diagnosis”. Bentham Science Publishers, Erasmus University Medical Center (Erasmus MC), Rotterdam, The Netherlands 2012. Disponible en: https://books.google.com/books?hl=es&lr=&id=HVrUb8DxKQkC&oi=fnd&pg=PP1&dq=The+role+of+new+technologies+in+medical+microbiological+research+and+diagnosis.+Bentham+Science+Publishers+2012.&ots=QTUPczGpZ-&sig=prsQJx2AFurreB1fR7bhH9qbGF0
S. Tejada, A. Romero and J. Rello. “Community-Acquired Pneumonia in Adults: What's New Focusing on Epidemiology, Microorganisms and Diagnosis?”, Erciyes Medical Journal, vol. 40, no. 4, pp. 177-182. https://doi.org/10.5152/etd.2018.18128 DOI: https://doi.org/10.5152/etd.2018.18128
C. D. Doern and M. Holfelder. “Automation and Design of the Clinical Microbiology Laboratory”. In Manual of Clinical Microbiology 11th edition, chapter 5. https://doi.org/10.1128/9781555817381.ch5 DOI: https://doi.org/10.1128/9781555817381.ch5
M. I. Sánchez-Romero, J. M. García-Lechuz Moya, J. J. González-López y N. Orta-Mira. “Recogida, transporte y procesamiento general de las muestras en el laboratorio de Microbiología”. Enfermedades Infecciosas y Microbiología Clínica, vol. 37, no. 2, pp. 127-134, Febrero 2019. https://doi.org/10.1016/j.eimc.2017.12.002 DOI: https://doi.org/10.1016/j.eimc.2017.12.002
D. J. Hardy. “Practical Aspects and Considerations When Planning a New Clinical Microbiology Laboratory”. Clinics in Laboratory Medicine, vol. 40, no. 4, pp. 421-431, Diciembre 2020. https://doi.org/10.1016/j.cll.2020.08.015 DOI: https://doi.org/10.1016/j.cll.2020.08.015
S. Novak and E. M. Marlowe. “Automation in the Clinical Microbiology Laboratory”. Clinics in Laboratory Medicine, vol. 33, no. 3, pp. 567–588, Septiembre 2013. https://doi.org/10.1016/j.cll.2013.03.002 DOI: https://doi.org/10.1016/j.cll.2013.03.002
E. Garcia, A. Ali and S. Choudhry. “The American Society for Clinical Pathology 2012 vacancy survey of clinical laboratories in the United States”. Laboratory Medicine, vol. 44, no. 1, pp. e1–e18, Febrero 2013. https://doi.org/10.1309/LMZK40NMGLFLW9SX DOI: https://doi.org/10.1309/LMZK40NMGLFLW9SX
T. H. Froland, I. Heldal, G. Sjoholt and E. Ersvær. “Games on mobiles via web or virtual reality technologies: How to support learning for biomedical laboratory science education”. Information, vol. 11, no. 4, pp. 195-210. https://doi.org/10.3390/info11040195 DOI: https://doi.org/10.3390/info11040195
C. P. Yeo and W. Y. Ng. “Automation and productivity in the clinical laboratory: experience of a tertiary healthcare facility”. Singapore medical journal, vol. 59, no. 11, pp. 597-601, Noviembre 2018. https://doi.org/10.11622%2Fsmedj.2018136 DOI: https://doi.org/10.11622/smedj.2018136
A. Croxatto, G. Prod'hom, F. Faverjon, Y. Rochais and G. Greub. “Laboratory automation in clinical bacteriology: what system to choose?”. Clinical Microbiology and Infection, vol. 22, no. 3, pp. 217–235. ISSN 1198-743X https://doi.org/10.1016/j.cmi.2015.09.030 DOI: https://doi.org/10.1016/j.cmi.2015.09.030
A. Croxatto, K. Dijkstra, G. Prod'hom and G. Greub. “Comparison of inoculation with the InoqulA and WASP automated systems with manual inoculation”. Journal of Clinical Microbiology, vol. 53, no. 7, pp. 2298-2307, Junio 2015. https://doi.org/10.1128/jcm.03076-14 DOI: https://doi.org/10.1128/JCM.03076-14
O. Dauwalder, L. Landrieve, F. Laurent, M. de Montclos, F. Vandenesch and G. Lina. “Does bacteriology laboratory automation reduce time to results and increase quality management?”, Clinical Microbiology and Infection, vol. 22, no. 3, pp. 236-243, Marzo 2016. ISSN 1198-743X, https://doi.org/10.1016/j.cmi.2015.10.037. DOI: https://doi.org/10.1016/j.cmi.2015.10.037
L. S. Garcia (ed.). Clinical microbiology procedures handbook. 3th edition. USA. American Society for Microbiology Press, 2010. Disponible en: https://books.google.com/books?hl=es&lr=&id=Fu2rBAAAQBAJ&oi=fnd&pg=PT8&dq=Clinical+microbiology+procedures+handbook&ots=p4AsuwlZJj&sig=HSpiVlcWk2l74rdHEe29gTX58NA
A. Mischnik, M. Trampe and S. Zimmermann. “Evaluation of the impact of automated specimen inoculation, using PREVI IsOLA, on the quality of and technical time for stool cultures”. Annals of Laboratory Medicine, vol. 35, no. 1, pp. 82–88. ISSN 2234-3806. https://doi.org/10.3343/alm.2015.35.1.82 DOI: https://doi.org/10.3343/alm.2015.35.1.82
Agentes fisicos. [Online]. Febrero 2005. Disponible en: https://www.ugr.es/~eianez/Microbiologia/13agfisicos.htm#_Toc59451620
M. Correa, M. Sabrina, F. Guacho, E. Villarreal y S. Valencia. “Desinfección mediante el uso de luz UV-C germicida en diferentes medios como estrategia preventiva ante la COVID-19”. Revista Minerva: Multidisciplinaria de Investigación Científica, vol. 1, no. 2, pp. 46-53. https://doi.org/10.47460/minerva.v1i2.11 DOI: https://doi.org/10.47460/minerva.v1i2.11
J. P. Sánchez, M. A. Echandi, J. A. Prada y D. Salas-Segura. “Luz ultravioleta germicida y control de microorganismos ambientales en hospitales”. Revista Costarricense de Salud Pública, San José, Costa Rica, vol. 21, no. 1, pp. 19-22. Disponible en: http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S1409-14292012000100005&lng=en&tlng=es
J. Nielsen and J. D. Keasling. “Engineering Cellular Metabolism”, Cell, vol. 164, no 6, pp. 1185-1197, Marzo 2016. https://doi.org/10.1016/j.cell.2016.02.004 DOI: https://doi.org/10.1016/j.cell.2016.02.004
D. Millán-Villarroel, L. Romero-González, M. Brito y A. Y. Ramos-Villarroel. “Luz ultravioleta: inactivación microbiana en frutas”. Saber, vol. 27, no. 3, pp. 454-469, Septiembre 2015. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S1315-01622015000300011&lng=es.
Edpilo (2020, Agosto 02). “Esterilizacion de objetos con UV”. [En línea] Disponible en: https://www.novomun.com/radiacion-uv-para
G. A. Acevedo Hernández, et al. “Sistema e-Salud para el monitoreo de un prototipo de incubadora neonatal”. Ciencia e Ingeniería, vol. 38, no 2, pp. 107-112. Universidad de los Andes, Venezuela. Disponible en: http://www.redalyc.org/articulo.oa?id=507555007002
F. P. Incropera and D. P. Dewitt. Fundamentos de transferencia de calor. Cuarta Edición, México, Pearson Educación, 1999. Disponible en: https://books.google.com/books?hl=es&lr=&id=QqfJw4tpIjcC&oi=fnd&pg=PA25&dq=Incropera,+F.+P.,+DeWitt,+D.+P.+(1999).+Fundamentos+de+transferencia+de+calor.+Espa%C3%B1a:+Pearson+Educaci%C3%B3n.&ots=p3LXsl8zOu&sig=dkOo6PMqpZAE6yoK0fsb1gAAdG0
D. Cera-Martínez, J. E. Ortiz-Sandoval y O. E. Gualdrón-Guerrero. “Sintonización de un controlador de temperatura a través de un autómata programable”. Revista De Investigación, Desarrollo E Innovación. vol. 9, no 1, pp. 177-186, 2018. https://doi.org/10.19053/20278306.v9.n1.2018.8513 DOI: https://doi.org/10.19053/20278306.v9.n1.2018.8513
K. Ogata (1996, enero 01). Sistemas de control en tiempo discreto. 2da ed. Pearson educación, 1996. Disponible en: https://books.google.com/books?hl=es&lr=&id=aYFUs17m0YQC&oi=fnd&pg=PA11&dq=Ogata,+K.+(1996).+Sistemas+de+control+en+tiempo+discreto.+Pearson+educaci%C3%B3n.&ots=5KcM-tSC4E&sig=FTUexHBaHT2L9J9WD83V-nn1YSQ
J. A. Dávalos-Pinto, E. Ávila-Córdova and C. B. Castillón-Lévano. “Design and implementation of a digital PID temperature controller for neonatal incubator ESVIN”. Journal of Mechanics Engineering and Automation, vol. 5, no. 3, pp. 167-172, Marzo 2015. http://doi.org/10.17265/2159-5275/2015.03.005 DOI: https://doi.org/10.17265/2159-5275/2015.03.005
W. Widhiada, I. N. G. Antara, I. N. Budiarsa and I. M. G. Karohika. “The robust PID control system of temperature stability and humidity on infant incubator based on Arduino at mega 2560”. In IOP Conference Series: Earth and Environmental Science, vol. 248, no. 1, pp. 1-12. IOP Publishing 2019. https://doi.org/10.1088/1755-1315/248/1/012046 DOI: https://doi.org/10.1088/1755-1315/248/1/012046
L. Xu, P. Deng, W. Song, M. Liu, M. Liu, M. Wang, Y. Yu and F. Wang. “An AIE-Active Cyclometalated Iridium (III) Photosensitizer for Selective Discrimination, Imaging, and Synergistic Elimination of Gram-Positive Bacteria”. ACS Materials Letters 2023, vol. 5, no. 1, pp. 162-171. https://doi.org/10.1021/acsmaterialslett.2c00774 DOI: https://doi.org/10.1021/acsmaterialslett.2c00774
T. D. Tavares, J. C. Antunes, J. Padrão, A. I. Ribeiro, A. Zille, M. T. P. Amorim and H. P. Felgueiras. “Activity of specialized biomolecules against Gram-positive and Gram-negative bacteria”. Antibiotics, vol. 9, no. 6, 314. https://doi.org/10.3390/antibiotics9060314 DOI: https://doi.org/10.3390/antibiotics9060314
A. Bârsan. “Position control of a mobile robot through PID controller”. Acta Universitatis Cibiniensis. Technical Series, vol. 71, no. 1, pp. 14-20. https://doi.org/10.2478/aucts-2019-0004 DOI: https://doi.org/10.2478/aucts-2019-0004
L. J. Montero-García, A. Castillo-González, O. Y. Chang-Espinosa y O. Torres-Monfil. “Desinfección de superficies mediante el empleo de luz UVC”. Innovación en Biotecnología I, pp. 75-84. ISBN 978-607-8617-85-2. Disponible en: https://redibai-myd.org/portal/wp-content/uploads/2021/01/607-8617-85-2.pdf#page=82
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