Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles

Authors

  • Idalia Yazmin Castañeda-Yslas Faculty of Sciences, Autonomous University of Baja California, KM. 106 Carr. Tijuana-Ensenada, 22800, Ensenada, Baja California, Mexico https://orcid.org/0000-0002-6754-0736
  • Balam Ruiz-Ruiz Faculty of Sciences, Autonomous University of Baja California, KM. 106 Carr. Tijuana-Ensenada, 22800, Ensenada, Baja California, Mexico https://orcid.org/0000-0003-2292-5891
  • María Evarista Arellano-García Faculty of Sciences, Autonomous University of Baja California, KM. 106 Carr. Tijuana-Ensenada, 22800, Ensenada, Baja California, Mexico https://orcid.org/0000-0002-0997-6902
  • Karla Oyuki Juárez-Moreno Center for Nanoscience and Nanotechnology, National Autonomous University of Mexico, Ensenada, Baja California, Mexico https://orcid.org/0000-0002-6171-8601
  • Olivia Torres-Bugarín Faculty of Medicine, Autonomous University of Guadalajara, Guadalajara, Jalisco, Mexico https://orcid.org/0000-0003-4541-8067
  • Nina Bogdanchikova Center for Nanoscience and Nanotechnology, National Autonomous University of Mexico, Ensenada, Baja California, Mexico
  • Alexey Pestryakov Tomsk Polytechnic University, 634050, Tomsk, Russia https://orcid.org/0000-0002-9034-4733
  • Patricia Radilla-Chávez Ensenada School of Medicine, Autonomous University of Baja California, Ensenada, Baja California, Mexico https://orcid.org/0000-0002-0496-4793

DOI:

https://doi.org/10.37636/recit.v126568

Keywords:

Silver Nanoparticles, Micronuclei, Reticulocytes, Genotoxicity.

Abstract

The purpose of this work was to evaluate the genotoxic capacity of AgNPs Argovit™ with the micronucleus technique in mouse peripheral blood reticulocytes. Argovit™ is administered orally in therapeutic doses (indicated for dogs as treatment for distemper) for three days to an experimental group with seven mice.  Another two groups with an equal number of mice were administered with injectable water and arabinose C, respectively. The specimens were measured and weighed before and after the experiment. Peripheral blood samples were also taken daily by a cut at the tip of the tail and then extended on slides for micronucleus test in reticulocytes. The smears were fixed in ethanol at 80 % and stained with acridine orange, after that, the presence of micronuclei was identified in 1000 reticulocytes counted by fluorescence microscopy. Preliminary results show significant differences in the number of micronuclei in reticulocytes between the three groups, so it’s necessary to compare this result with another type of in vitro models.

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Micronucleated polychromatic erythrocytes in 1000 polychromatic erythrocytes

Published

2018-01-01

How to Cite

Castañeda-Yslas, I. Y., Ruiz-Ruiz, B., Arellano-García, M. E., Juárez-Moreno, K. O., Torres-Bugarín, O., Bogdanchikova, N., Pestryakov, A., & Radilla-Chávez, P. (2018). Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles. REVISTA DE CIENCIAS TECNOLÓGICAS, 1(2), 65–68. https://doi.org/10.37636/recit.v126568

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