Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles

Authors

DOI:

https://doi.org/10.37636/recit.v126568

Keywords:

Silver Nanoparticles, Micronuclei, Reticulocytes, Genotoxicity.

Abstract

The purpose of this work was to evaluate the genotoxic capacity of AgNPs Argovit™ with the micronucleus technique in mouse peripheral blood reticulocytes. Argovit™ is administered orally in therapeutic doses (indicated for dogs as treatment for distemper) for three days to an experimental group with seven mice.  Another two groups with an equal number of mice were administered with injectable water and arabinose C, respectively. The specimens were measured and weighed before and after the experiment. Peripheral blood samples were also taken daily by a cut at the tip of the tail and then extended on slides for micronucleus test in reticulocytes. The smears were fixed in ethanol at 80 % and stained with acridine orange, after that, the presence of micronuclei was identified in 1000 reticulocytes counted by fluorescence microscopy. Preliminary results show significant differences in the number of micronuclei in reticulocytes between the three groups, so it’s necessary to compare this result with another type of in vitro models.

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References

B. Nowack, H. F. Krug, y M. Height, "120 years of nanosilver history: Implications for policy makers", Environ. Sci. Technol., vol. 45, núm. 4, pp. 1177-1183, 2011. https://doi.org/10.1021/es103316q DOI: https://doi.org/10.1021/es103316q

L. Ge, Q. Li, M. Wang, J. Ouyang, X. Li, y M. M. Q. Xing, "Nanosilver particles in medical applications: Synthesis, performance, and toxicity", International Journal of Nanomedicine, vol. 9, núm. 1. pp. 2399-2407, 2014. https://doi.org/10.2147/IJN.S55015 DOI: https://doi.org/10.2147/IJN.S55015

Y. Li et al., "Genotoxicity of silver nanoparticles evaluated using the Ames test and in vitro micronucleus assay", Mutat. Res. - Genet. Toxicol. Environ. Mutagen., vol. 745, núm. 1-2, pp. 4-10, 2012. https://doi.org/10.1016/j.mrgentox.2011.11.010 DOI: https://doi.org/10.1016/j.mrgentox.2011.11.010

A. G. Glotov, T. I. Glotova, A. A. Sergeev, T. V Belkina, y A. N. Sergeev, "[Antiviral activity of different drugs in vitro against viruses of bovine infectious rhinotracheitis and bovine diarrhea]", Vopr Virusol, vol. 49, núm. 5, pp. 43-46, 2004. https://pubmed.ncbi.nlm.nih.gov/15529864/

F. V. Semenov y K. M. Fidarova, "[The treatment of the patients presenting with chronic inflammation of the trepanation cavity with a preparation containing silver nanoparticles following sanitation surgery of the open type]", Vestn. Otorinolaringol., núm. 6, pp. 117-119, 2012. https://pubmed.ncbi.nlm.nih.gov/23268263/

N. Bogdanchikova et al., "Silver nanoparticles composition for the treatment of distemper in dogs", Int. J. Nanotechnol., vol. 13, núm. 1/2/3, p. 227, 2016. https://doi.org/10.1504/IJNT.2016.074536 DOI: https://doi.org/10.1504/IJNT.2016.074536

B. Borrego et al., "Potential application of silver nanoparticles to control the infectivity of Rift Valley fever virus in vitro and in vivo", Nanomedicine Nanotechnology, Biol. Med., vol. 12, núm. 5, pp. 1185-1192, 2016. https://doi.org/10.1016/j.nano.2016.01.021 DOI: https://doi.org/10.1016/j.nano.2016.01.021

O. V. Kalmantaeva et al., "Silver-nanoparticle exposure on the immune system of mice depending on the route of administration", Nanotechnologies Russ., vol. 9, núm. 9-10, pp. 571-576, 2014. https://doi.org/10.1134/S1995078014050061 DOI: https://doi.org/10.1134/S1995078014050061

K. Juarez-Moreno et al., "Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines.", Hum. Exp. Toxicol., p. 960327116675206, 2016. DOI: https://doi.org/10.1177/0960327116675206

https://doi.org/10.1177/0960327116675206 DOI: https://doi.org/10.1177/0960327116675206

Committee, Guide for the Care and Use of Laboratory Animals: Eighth Edition. 2011. https://www.ncbi.nlm.nih.gov/books/NBK54050/

Micronucleated polychromatic erythrocytes in 1000 polychromatic erythrocytes

Published

2018-01-01

How to Cite

Castañeda-Yslas, I. Y., Ruiz-Ruiz, B., Arellano-García, M. E., Juárez-Moreno, K. O., Torres-Bugarín, O., Bogdanchikova, N., … Radilla-Chávez, P. (2018). Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles. Revista De Ciencias Tecnológicas, 1(2), 65–68. https://doi.org/10.37636/recit.v126568

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