Preparation of Mucoadhesive Films of Polyelectrolyte Complexes for the Release of Chlorhexidine Diacetate
DOI:
https://doi.org/10.37636/recit.v311022Keywords:
Polyelectrolyte films, Mucoadhesion, Chlorhexidine diacetate.Abstract
Polyelectrolyte complexe’s films can be a vehicle for the controlled release of drugs in the oral cavity, seeking the interaction of the film with the epithelium of the buccal mucosa. The bioadhesion phenomenon is the retention of the system at the desired site to achieve liberation. In the present work, a release system was developed using polyelectrolyte polymers of opposite charge that form films by ionic complexation. The films were loaded with chlorhexidine diacetate for the treatment of oral infections. The solvent evaporation method was used for the preparation of films, containing arabic gum and chitosan (1: 1) and Noveon AA-1® 2% (w/v), which was included for its mucoadhesive properties. The films were prepared with the same proportions of polymers but adding chlorhexidine diacetate dissolved in ethanol 2% (w/v), before the evaporation of the solvent for the formation of the films. Swelling studies shows that films swell in solutions that simulate oral pH conditions. The films loaded with the drug release in vitro more than 90% of the drug in 24 hours. Microbiological tests showed that films loaded with chlorhexidine diacetate inhibit the growth of E. coli and P. aeruginosa in standardized sensitivity tests. The films studied show suitable characteristics for the release of chlorhexidine diacetate, demonstrating the inhibition of the growth of pathogenic microorganisms, so they have potential to be used in the treatment of patients with oral infections such as gingivitis and periodontal disease.Downloads
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Y. Sudhakar, K. Kuotsu, and A. K. Bandyopadhyay, "Buccal bioadhesive drug delivery - A promising option for orally less efficient drugs," J. Control. Release, vol. 114, pp. 15-40, 2006. https://doi.org/10.1016/j.jconrel.2006.04.012. DOI: https://doi.org/10.1016/j.jconrel.2006.04.012
S. Mansuri, P. Kesharwani, K. Jain, R. K. Tekade, and N. K. Jain, "Mucoadhesion: A promising approach in drug delivery system," Reactive and Functional Polymers, vol. 100. pp. 151-172, 2016. https://doi.org/10.1016/j.reactfunctpolym.2016.01.011. DOI: https://doi.org/10.1016/j.reactfunctpolym.2016.01.011
J. das Neves, R. Nunes, A. Machado, and B. Sarmento, "Polymer-based nanocarriers for vaginal drug delivery," Adv. Drug Deliv. Rev., vol. 92, pp. 53-70, 2015. https://doi.org/10.1016/j.addr.2014.12.004. DOI: https://doi.org/10.1016/j.addr.2014.12.004
J. Saurí et al., "Formulation and characterization of mucoadhesive controlled release matrix tablets of captopril," J. Drug Deliv. Sci. Technol., vol. 42, no. 17, pp. 215-226, 2017. https://doi.org/10.1016/j.jddst.2017.03.009. DOI: https://doi.org/10.1016/j.jddst.2017.03.009
K. D. Madsen, C. Sander, S. Baldursdottir, A. M. L. Pedersen, and J. Jacobsen, "Development of an ex vivo retention model simulating bioadhesion in the oral cavity using human saliva and physiologically relevant irrigation media," Int. J. Pharm., vol. 448, no. 2, pp. 373-381, 2013. https://doi.org/10.1016/j.ijpharm.2013.03.031. DOI: https://doi.org/10.1016/j.ijpharm.2013.03.031
S. Salehi and S. Boddohi, "Design and optimization of kollicoat ® IR based mucoadhesive buccal film for co-delivery of rizatriptan benzoate and propranolol hydrochloride," Mater. Sci. Eng. C, vol. 97, no. June 2018, pp. 230-244, 2019. https://doi.org/10.1016/j.msec.2018.12.036. DOI: https://doi.org/10.1016/j.msec.2018.12.036
R. Pignatello, A. H. S. Stancampiano, C. A. Ventura, and G. Puglisi, "Dexamethasone sodium phosphate-loaded chitosan based delivery systems for buccal application.," J. Drug Target., vol. 15, no. 9, pp. 603-610, 2007. https://doi.org/10.1080/10611860701502806. DOI: https://doi.org/10.1080/10611860701502806
H. O. Ammar, M. M. Ghorab, A. A. Mahmoud, and H. I. Shahin, "Design and In Vitro/In Vivo Evaluation of Ultra-Thin Mucoadhesive Buccal Film Containing Fluticasone Propionate," AAPS PharmSciTech, vol. 18, no. 1, 2016. https://doi.org/10.1208/s12249-016-0496-0. DOI: https://doi.org/10.1208/s12249-016-0496-0
M. L. B. Palacio and B. Bhushan, "Bioadhesion: a review of concepts and applications Bioadhesion: a review of concepts and applications," Phil. Trans. R. Soc. A, vol. 370, pp. 2321–2347 2012. https://doi.org/10.1098/rsta.2011.0483. DOI: https://doi.org/10.1098/rsta.2011.0483
N. A. Nafee, F. A. Ismail, N. A. Boraie, and L. M. Mortada, "Mucoadhesive delivery systems. I. Evaluation of mucoadhesive polymers for buccal tablet formulation.," Drug Dev. Ind. Pharm., vol. 30, no. 9, pp. 985-993, 2004. https://doi.org/10.1081/DDC-200037245. DOI: https://doi.org/10.1081/DDC-200037245
C. K. Mylangam, S. Beeravelli, J. Medikonda, and J. S. Pidaparthi, "Badam gum: a natural polymer in mucoadhesive drug delivery. Design, optimization, and biopharmaceutical evaluation of badam gum-based metoprolol succinate buccoadhesive tablets," vol. 7544, pp. 1-12, 2014. https://doi.org/10.3109/10717544.2014.908979. DOI: https://doi.org/10.3109/10717544.2014.908979
Y. Luo and Q. Wang, "Recent development of chitosan-based polyelectrolyte complexes with natural polysaccharides for drug delivery," Int. J. Biol. Macromol., vol. 64, pp. 353-367, 2014. https://doi.org/10.1016/j.ijbiomac.2013.12.017. DOI: https://doi.org/10.1016/j.ijbiomac.2013.12.017
A. Abruzzo et al., "Chitosan / alginate complexes for vaginal delivery of chlorhexidine digluconate," Carbohydr. Polym., vol. 91, no. 2, pp. 651-658, 2013. https://doi.org/10.1016/j.carbpol.2012.08.074. DOI: https://doi.org/10.1016/j.carbpol.2012.08.074
H. Espinosa-Andrews, J. G. Báez-González, F. Cruz-Sosa, and E. J. Vernon-Carter, "Gum arabic-chitosan complex coacervation," Biomacromolecules, vol. 8, no. 4, pp. 1313-1318, 2007. https://doi.org/10.1021/bm0611634. DOI: https://doi.org/10.1021/bm0611634
D. Sakloetsakun, D. Preechagoon, A. Bernkop-schnu, and T. Pongjanyakul, "Chitosan - gum arabic polyelectrolyte complex films: physicochemical, mechanical and mucoadhesive properties," vol. 7450, pp. 1-10, 2015. https://doi.org/10.3109/10837450.2015.1035727. DOI: https://doi.org/10.3109/10837450.2015.1035727
R. Tsai et al., "Chitosan / pectin / gum Arabic polyelectrolyte complex: Process-dependent appearance, microstructure analysis and its application," Carbohydr. Polym., vol. 101, pp. 752-759, 2014. https://doi.org/10.1016/j.carbpol.2013.10.008. DOI: https://doi.org/10.1016/j.carbpol.2013.10.008
A. A. Kassem, F. A. Ismail, V. F. Naggar, and E. Aboulmagd, "Preparation and evaluation of periodontal films based on polyelectrolyte complex formation," vol. 7450, no. Na CMC, pp. 1-9, 2014.
L. Perioli et al., "Novel mucoadhesive buccal formulation containing metronidazole for the treatment of periodontal disease," J. Control. Release, vol. 95, no. 3, pp. 521-533, 2004. https://doi.org/10.1016/j.jconrel.2003.12.018. DOI: https://doi.org/10.1016/j.jconrel.2003.12.018
V. Ambrogi, L. Perioli, F. Marmottini, M. Moretti, E. Lollini, and C. Rossi, "Chlorhexidine MCM-41 Mucoadhesive Tablets for Topical Use," pp. 156-164, 2009. https://doi.org/10.1007/s12247-009-9073-3. DOI: https://doi.org/10.1007/s12247-009-9073-3
"M44-A2: Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts; Approved Guideline-Second Edition," no. August, 2009. https://clsi.org/media/1634/m44a2_sample.pdf.
S. Testing, M100-S23 Performance Standards for Antimicrobial, no. January. 2013. https://www.facm.ucl.ac.be/intranet/CLSI/CLSI-M100S23-susceptibility-testing-2013-no-protection.pdf.
C. Woertz, M. Preis, J. Breitkreutz, and P. Kleinebudde, "Assessment of test methods evaluating mucoadhesive polymers and dosage forms: An overview," Eur. J. Pharm. Biopharm., vol. 85, no. 3 PART B, pp. 843-853, 2013. https://doi.org/10.1016/j.ejpb.2013.06.023. DOI: https://doi.org/10.1016/j.ejpb.2013.06.023
J. O. Morales and J. T. McConville, "Manufacture and characterization of mucoadhesive buccal films," Eur. J. Pharm. Biopharm., vol. 77, no. 2, pp. 187-199, 2011. https://doi.org/10.1016/j.ejpb.2010.11.023. DOI: https://doi.org/10.1016/j.ejpb.2010.11.023
C. Juliano, M. Cossu, P. Pigozzi, G. Rassu, and P. Giunchedi, "Preparation, in vitro characterization and preliminary in vivo evaluation of buccal polymeric films containing chlorhexidine," AAPS PharmSciTech, vol. 9, no. 4, pp. 1153-1158, 2008. https://doi.org/10.1208/s12249-008-9153-6. DOI: https://doi.org/10.1208/s12249-008-9153-6
N. A. Peppas and P. A. Buri, "Surface, interfacial and molecular aspects of polymer bioadhesion on soft tissues," J. Control. Release, vol. 2, pp. 257-275, 1985. https://doi.org/10.1016/0168-3659(85)90050-1. DOI: https://doi.org/10.1016/0168-3659(85)90050-1
J. D. Smart, "The basics and underlying mechanisms of mucoadhesion," Adv. Drug Deliv. Rev., vol. 57, no. 11, pp. 1556-1568, 2005. https://doi.org/10.1016/j.addr.2005.07.001. DOI: https://doi.org/10.1016/j.addr.2005.07.001
R. Shaikh, T. R. Raj Singh, M. J. Garland, a D. Woolfson, and R. F. Donnelly, "Mucoadhesive drug delivery systems.," J. Pharm. Bioallied Sci., vol. 3, no. 1, pp. 89-100, 2011. https://doi.org/10.4103/0975-7406.76478. DOI: https://doi.org/10.4103/0975-7406.76478
C. Eouani, P. Piccerelle, P. Prinderre, E. Bourret, and J. Joachim, "In-vitro comparative study of buccal mucoadhesive performance of different polymeric films," Eur. J. Pharm. Biopharm., vol. 52, no. 1, pp. 45-55, 2001. https://doi.org/10.1016/S0939-6411(01)00146-1. DOI: https://doi.org/10.1016/S0939-6411(01)00146-1
S. A. Mortazavi, D. Delil, and B. Sciences, "An investigation of some factors influencing the in vitro assessment of mucoadhesion," Int. J. Pharm., vol. 116, pp. 223-230, 1995. https://doi.org/10.1016/0378-5173(94)00299-K. DOI: https://doi.org/10.1016/0378-5173(94)00299-K
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Copyright (c) 2020 Ayla Carolina Vea-Barragán, Nydia Alejandra Castillo-Martínez, Aracely Serrano-Medina, Eduardo Alberto López-Maldonado, Samuel Guillermo Meléndez-López, José Manuel Cornejo-Bravo
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