Evaluación de toxicidad de nanogeles termosensibles en modelo in vivo

Alondra Montañez Rios; Aracely Serrano Medina; Juan M. Irache; Ana Luisa  Martínez López; Ignacio Alfredo  Rivero Espejel; Jose Manuel  Cornejo Bravo

doi:10.37636/recit.v5n3e236

Authors

Alondra Montañez Rios Universidad Autónoma de Baja California, Facultad de Ciencias Químicas e Ingeniería, Calzada Universidad 14418, Parque industrial Internacional, Tijuana, Baja California, México, C.P. 22427. https://orcid.org/0000-0001-6784-9605
Aracely Serrano Medina Universidad Autónoma de Baja California, Facultad de Medicina y Psicología, Calzada Universidad 14418, Parque industrial Internacional, Tijuana, Baja California, México, C.P. 22427.
Juan M. Irache NANO-VAC Research Group, Department of Chemistry and Pharmaceutical Technology, University of Navarra, Pamplona 31080, Spain. https://orcid.org/0000-0001-9687-3436
Ana Luisa Martínez López NANO-VAC Research Group, Department of Chemistry and Pharmaceutical Technology, University of Navarra, Pamplona 31080, Spain.
Ignacio Alfredo Rivero Espejel Tecnológico Nacional de México/I. T. Tijuana. Centro de Graduados e Investigación en Química, Blvd. Alberto Limón Padilla S/N, 22510 Tijuana, Baja California, México https://orcid.org/0000-0003-4920-6379
Jose Manuel Cornejo Bravo Universidad Autónoma de Baja California, Facultad de Ciencias Químicas e Ingeniería, Calzada Universidad 14418, Parque industrial Internacional, Tijuana, Baja California, México, C.P. 22427. https://orcid.org/0000-0002-0013-8937

DOI:

https://doi.org/10.37636/recit.v5n3e236

Keywords:

Nanogels, C. elegans, NIPAAm, Toxicity

Abstract

This study uses C. elegans to assess the toxicity of copolymeric nanogels (NG) of N-isopropylacrylamide (NIPAAm) and 2-(diethylamino)ethyl methacrylate (DEAEM), prepared by surfactant-free emulsion polymerization using polyethylene glycol monomethyl ether methacrylate (PEGMA) as a reactive stabilizer. Nematodes ingested fluorescein-labeled NG, as demonstrated by fluorescence microscopy. Two different initiators, cationic and anionic, were used to initiate the synthesis of the NG. The results indicate that both types of NG affect the size and reproducibility of nematodes. C. elegans is a potential multicellular model to evaluate the toxicity of sensitive NG, avoiding the use of mammals for evaluations.

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