Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles
Keywords:Silver Nanoparticles, Micronuclei, Reticulocytes, Genotoxicity.
AbstractThe purpose of this work was to evaluate the genotoxic capacity of AgNPs Argovit™ with the micronucleus technique in mouse peripheral blood reticulocytes. Argovit™ is administered orally in therapeutic doses (indicated for dogs as treatment for distemper) for three days to an experimental group with seven mice. Another two groups with an equal number of mice were administered with injectable water and arabinose C, respectively. The specimens were measured and weighed before and after the experiment. Peripheral blood samples were also taken daily by a cut at the tip of the tail and then extended on slides for micronucleus test in reticulocytes. The smears were fixed in ethanol at 80 % and stained with acridine orange, after that, the presence of micronuclei was identified in 1000 reticulocytes counted by fluorescence microscopy. Preliminary results show significant differences in the number of micronuclei in reticulocytes between the three groups, so it’s necessary to compare this result with another type of in vitro models.
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Copyright (c) 2018 Castañeda-Yslas Idalia Yazmin, Ruiz-Ruiz Balam, Arellano-García María Evarista, Juárez-Moreno Karla Oyuki, Torres-Bugarín Olivia, Bogdanchikova Nina, Pestryakov Alexey, Radilla-Chávez Patricia
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